Protective effects of ursolic acid on cyclophosphamide induced hemorrhagic cystitis in rats

Cyclophosphamide (CP) is a commonly used antineoplastic agent despite its adverse effects. Hemorrhagic cystitis (HC)is one of the most common side effects CP. Although Mesna to prevent HC, it suffers from few sideeffects. Therefore, search for better alternate substance still continues. In this study, we studied ursolic acid(UA), active ingredients Plantago major, called plantain or white man’s foot, in CP-inducedhemorrhagic rats. Forty-nine Sprague−Dawley rats seven equal groups viz. control (C); cyclophosphamide(CP); cyclophosphamide+Mesna (CPMesna); cyclophosphamide+ursolic acid [100 (CPUA100) and 200 mg (CPUA200)],and cyclophosphamide+ mesna+ (100, (CPMesnaUA100) (200 (CPMesnaUA200) were investigated forinducible nitric oxide synthetase (iNOs), total antioxidant status (TAS), (TOS), TNF–α, interleukin10 (IL–10) interleukin 6 (IL–6) levels blood samples. The data obtained statistically analyzed using SPSSand both Kruskall Wallis Dunn tests performed. Value P <0.05 was considered as significant. Ourresults have revealed that UA applied preserve compared CP group. However, observed thisprotection comparatively weaker than UA-Mesna combinations. Hence, suggested urosilicacid could cyclophosphamide induced hemorrhagic cystitis, also extends support applications.Overall, has been limited protective against CP-induced andcan serve supportive inhibiting cystitis.